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Application of a novel continuous sampler for the determination of compounds in ultrafine aerosol
Hlaváčková, Hana ; Řezáčová, Veronika (referee) ; Alexa,, Lukáš (advisor)
The diploma thesis is focused on the use of new type of continuous sampler for the determination of compounds in ultra-fine aerosol. The aim of the study was to optimize parameters of the aerosol sampler CGU-ACTJU (Condensation-Growth Unit – Aerosol Counterflow Two-Jets Unit) and its application for the real aerosol samples. The ACTJU sampler connected to upstreamed CGU enables sampling of ultra-fine fraction of particulate matter to the diameter about few nanometers. The measured results from CGU-ACTJU were compared with commercial sampler (cascade impactor) with subsequent offline analysis. The atmospheric aerosol was sampled in two campaigns (July–August 2021 and February 2022) on the terrace of Institute of Analytical Chemistry of CAS in Brno. Theoretical part of the study contains information about analytical methods for the characterization of aerosol, the experimental part describes used methods in detail and the results of experiments are discussed.
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Research on the release of nitrogen compounds from fertilizers
Cápková, Viola ; Řezáčová, Veronika (referee) ; Komendová, Renata (advisor)
This bachelor thesis deals with the monitoring of the release kinetics of ammonium, nitrate and phosphate ions from slow release fertilizers covered in potassium polyacrylate. Fertilizer samples were subjected to different extraction reagents and experimental conditions. The effect of dynamic and pressure conditions in comparison to the static conditions was investigated, same as exposion to distilled, drinking and atmospheric water. The change in concentration of each analyte was measured at regular intervals using molecular spectrophotometry. Distilled water was found to be the best extraction reagent, with no significant difference between the experimental conditions. Slow release of nutrients was proved for two fertilizer samples, the urea fertilizer and it’s modified form.
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Parallel Determination of Particulate Ammonium by Using a Continuous Aerosol Sampler and Cascade Impactor
Alexa, Lukáš ; Hlaváčková, H. ; Cigánková, Hana ; Mikuška, Pavel
Ammonia is a significant gaseous pollutant present in the atmosphere. As a neutralizing agent of acidic species ammonia forms particle-phase ammonium (NH4+) salts and contributes thus to formation of secondary atmospheric aerosols (Harrison and Jones, 1995). Aerosols (particulate matter, PM) are responsible for many negative effects on environments and health risks to human. The diameter of PM has significant impact on their environmental exposure, where the decreasing particle size increases the adverse health effects. The ultrafine particles hence play a major role in adverse impact on human health (Ryer-Powder, 1991). Presented paper describes the use of the novel continuous aerosol sampler for online, and a commercial cascade impactor for offline, determination of NH4+ in ambient air.
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Application of a novel continuous sampler for the determination of compounds in ultrafine aerosol
Hlaváčková, Hana ; Řezáčová, Veronika (referee) ; Alexa,, Lukáš (advisor)
The diploma thesis is focused on the use of new type of continuous sampler for the determination of compounds in ultra-fine aerosol. The aim of the study was to optimize parameters of the aerosol sampler CGU-ACTJU (Condensation-Growth Unit – Aerosol Counterflow Two-Jets Unit) and its application for the real aerosol samples. The ACTJU sampler connected to upstreamed CGU enables sampling of ultra-fine fraction of particulate matter to the diameter about few nanometers. The measured results from CGU-ACTJU were compared with commercial sampler (cascade impactor) with subsequent offline analysis. The atmospheric aerosol was sampled in two campaigns (July–August 2021 and February 2022) on the terrace of Institute of Analytical Chemistry of CAS in Brno. Theoretical part of the study contains information about analytical methods for the characterization of aerosol, the experimental part describes used methods in detail and the results of experiments are discussed.
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Ammmonium transport in yeast
Faltýnková, Kateřina ; Palková, Zdena (advisor) ; Princová, Jarmila (referee)
Bachelor thesis - Kateřina Faltýnková - Ammmonium transport in yeast Ammonium and ammonia are an essential nutrient for every yeast cells, not only in metabolism, for example in amino acid synthesis, but also as signalling molecules that serve for communication between colonies or for the regulation of pseudohyphal growth. Transport of ammonia and ammonium ions requires active transport, which is provided by MEP permeases inside the cell likely by exporters ATO proteins out of the cell. In this work there are described families of genes MEP and ATO with main focus on their importance for uptake and export of ammonium ions by yeast and also the regulation of these two gene families in the yeast Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans a Yarrowia lipolytica.
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Ammmonium transport in yeast
Faltýnková, Kateřina ; Palková, Zdena (advisor) ; Princová, Jarmila (referee)
Bachelor thesis - Kateřina Faltýnková - Ammmonium transport in yeast Ammonium and ammonia are an essential nutrient for every yeast cells, not only in metabolism, for example in amino acid synthesis, but also as signalling molecules that serve for communication between colonies or for the regulation of pseudohyphal growth. Transport of ammonia and ammonium ions requires active transport, which is provided by MEP permeases inside the cell likely by exporters ATO proteins out of the cell. In this work there are described families of genes MEP and ATO with main focus on their importance for uptake and export of ammonium ions by yeast and also the regulation of these two gene families in the yeast Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans a Yarrowia lipolytica.
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